micro guard cation h pre-column Search Results


final cation exchange chromatography step  (Repligen Corp)
95
Repligen Corp final cation exchange chromatography step
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Final Cation Exchange Chromatography Step, supplied by Repligen Corp, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/final cation exchange chromatography step/product/Repligen Corp
Average 95 stars, based on 1 article reviews
final cation exchange chromatography step - by Bioz Stars, 2026-03
95/100 stars
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cation exchange hitrap high performance sepharose hp sp column  (GE Healthcare)
92
GE Healthcare cation exchange hitrap high performance sepharose hp sp column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Cation Exchange Hitrap High Performance Sepharose Hp Sp Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cation exchange hitrap high performance sepharose hp sp column/product/GE Healthcare
Average 92 stars, based on 1 article reviews
cation exchange hitrap high performance sepharose hp sp column - by Bioz Stars, 2026-03
92/100 stars
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strong cation amersham biosciences exchange pre loaded column se53 cellulose  (Danaher Inc)
86
Danaher Inc strong cation amersham biosciences exchange pre loaded column se53 cellulose
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Strong Cation Amersham Biosciences Exchange Pre Loaded Column Se53 Cellulose, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/strong cation amersham biosciences exchange pre loaded column se53 cellulose/product/Danaher Inc
Average 86 stars, based on 1 article reviews
strong cation amersham biosciences exchange pre loaded column se53 cellulose - by Bioz Stars, 2026-03
86/100 stars
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hiscreen sp hp pre-packed chromatography column  (Danaher Inc)
90
Danaher Inc hiscreen sp hp pre-packed chromatography column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Hiscreen Sp Hp Pre Packed Chromatography Column, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hiscreen sp hp pre-packed chromatography column/product/Danaher Inc
Average 90 stars, based on 1 article reviews
hiscreen sp hp pre-packed chromatography column - by Bioz Stars, 2026-03
90/100 stars
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a column and pre-column (metrosep cation 1–2, 6.1010.000)  (Metrohm AG)
90
Metrohm AG a column and pre-column (metrosep cation 1–2, 6.1010.000)
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
A Column And Pre Column (Metrosep Cation 1–2, 6.1010.000), supplied by Metrohm AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a column and pre-column (metrosep cation 1–2, 6.1010.000)/product/Metrohm AG
Average 90 stars, based on 1 article reviews
a column and pre-column (metrosep cation 1–2, 6.1010.000) - by Bioz Stars, 2026-03
90/100 stars
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micro guard cation h pre-column  (Bio-Rad)
90
Bio-Rad micro guard cation h pre-column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Micro Guard Cation H Pre Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/micro guard cation h pre-column/product/Bio-Rad
Average 90 stars, based on 1 article reviews
micro guard cation h pre-column - by Bioz Stars, 2026-03
90/100 stars
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polysulfoethyl  (PolyLC INC)
90
PolyLC INC polysulfoethyl
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Polysulfoethyl, supplied by PolyLC INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polysulfoethyl/product/PolyLC INC
Average 90 stars, based on 1 article reviews
polysulfoethyl - by Bioz Stars, 2026-03
90/100 stars
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biorad pre column  (Bio-Rad)
93
Bio-Rad biorad pre column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Biorad Pre Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biorad pre column/product/Bio-Rad
Average 93 stars, based on 1 article reviews
biorad pre column - by Bioz Stars, 2026-03
93/100 stars
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metrosep c4 pre-column  (Metrohm AG)
90
Metrohm AG metrosep c4 pre-column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Metrosep C4 Pre Column, supplied by Metrohm AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/metrosep c4 pre-column/product/Metrohm AG
Average 90 stars, based on 1 article reviews
metrosep c4 pre-column - by Bioz Stars, 2026-03
90/100 stars
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polysulfoethyl a (4.6 x 200mm i.d.) cation exchange (cex) column  (PolyLC INC)
90
PolyLC INC polysulfoethyl a (4.6 x 200mm i.d.) cation exchange (cex) column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Polysulfoethyl A (4.6 X 200mm I.D.) Cation Exchange (Cex) Column, supplied by PolyLC INC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polysulfoethyl a (4.6 x 200mm i.d.) cation exchange (cex) column/product/PolyLC INC
Average 90 stars, based on 1 article reviews
polysulfoethyl a (4.6 x 200mm i.d.) cation exchange (cex) column - by Bioz Stars, 2026-03
90/100 stars
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ag 50w x8 cation exchange resin column  (Bio-Rad)
96
Bio-Rad ag 50w x8 cation exchange resin column
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Ag 50w X8 Cation Exchange Resin Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ag 50w x8 cation exchange resin column/product/Bio-Rad
Average 96 stars, based on 1 article reviews
ag 50w x8 cation exchange resin column - by Bioz Stars, 2026-03
96/100 stars
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stratatm xc polymeric strong cation solid phase extraction columns  (Danaher Inc)
86
Danaher Inc stratatm xc polymeric strong cation solid phase extraction columns
Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange <t>chromatography</t> in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.
Stratatm Xc Polymeric Strong Cation Solid Phase Extraction Columns, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange chromatography in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.

Journal: Biotechnology progress

Article Title: Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance.

doi: 10.1002/btpr.2805

Figure Lengend Snippet: Figure 1. Outline of experimental set-up. In the first case study, mAb 1 samples from a 50 L bioreactor run were taken at six points during the cultivation. Samples were centrifuged, filtered and then protein A purified, before being quantified for HCPs. In the second study, a DoE with mAb 2 was carried out to explore two cell culture parameters in order to determine the optimal upstream process conditions for high titer and low HCP levels. In the third study, mAb 3 was grown in shake flasks under fixed upstream parameters and cell broth samples were taken at four time points. At each time point, half of the material was sheared (2) while the other half of the material was left non-sheared (1). Both materials were subsequently centrifuged, filtered, and protein A purified. Next, all protein A eluate samples were further processed by anion exchange chromatography in flow-through mode and by cation exchange chromatography. All samples were analysed for product quality by measuring monomer percentage and HCP levels. Figure 2. Effects of harvest time on product titer and HCP levels (Case Study I). Increasing product amount over the course of a cultivation is accompanied by decreasing product purity. Supernatant samples were taken throughout the 50 L STR production bioreactor run of mAb 1 (including on harvest day) and antibody concentration was measured and normalised to 1 due to confidentiality. Supernatant samples were filtered, protein A purified, and analysed for HCP quantities by ELISA. Note that the HCP data points for days 1, 3 and 7 are estimated on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on assay serial dilutions and show one standard deviation. Figure 3. Effects of culture conditions (Case Study II). (A) Experimental DoE points. 14 fed- batch cultures were grown in 250 mL small scale bioreactor vessels (TAP ambr250) under a range of different pHs and temperatures. The empty, half-filled and filled symbols represent low, medium and high temperatures respectively, whereas the squares, triangles and circles represent low, medium and high pH points. All cultures were harvested on day 16. (B) Harvested samples were centrifuged, filtered and protein A purified, in order to determine HCP quantities, and correlate HCP data to titer results. Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (C) Peak cell densities and cell- specific productivity rates were determined for each sample. Highlighted in the grey oval are cultures associated with low titers.

Article Snippet: The flow-through material was collected and polished with a final cation exchange chromatography step, using 1 mL MiniChrom columns (Repligen, Waltham, MA) and a buffer with a pH below the pI of the protein.

Techniques: Purification, Cell Culture, Chromatography, Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

Figure 7. Effects of shear damage on product quality in early stage cell culture samples (Case Study III). Material from day 10 was processed as described in the legend for figure 1 (non- sheared vs. sheared) and aggregate / fragment levels were determined by analytical SEC after protein A purification. Figure 8. Effects of a polishing chromatography step on product quality (Case Study III). (A) HCP levels in non-sheared samples before and after cation exchange chromatography, as determined by ELISA. HCP data point for day 10 after CEX is approximated based on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (B) Fragmentation percentages in non- sheared and sheared samples before and after cation exchange chromatography, as determined by analytical SEC.

Journal: Biotechnology progress

Article Title: Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance.

doi: 10.1002/btpr.2805

Figure Lengend Snippet: Figure 7. Effects of shear damage on product quality in early stage cell culture samples (Case Study III). Material from day 10 was processed as described in the legend for figure 1 (non- sheared vs. sheared) and aggregate / fragment levels were determined by analytical SEC after protein A purification. Figure 8. Effects of a polishing chromatography step on product quality (Case Study III). (A) HCP levels in non-sheared samples before and after cation exchange chromatography, as determined by ELISA. HCP data point for day 10 after CEX is approximated based on titer as HCP levels were below detection (< 2 ng/mL). Descriptive error bars are based on HCP assay serial dilutions and show one standard deviation. (B) Fragmentation percentages in non- sheared and sheared samples before and after cation exchange chromatography, as determined by analytical SEC.

Article Snippet: The flow-through material was collected and polished with a final cation exchange chromatography step, using 1 mL MiniChrom columns (Repligen, Waltham, MA) and a buffer with a pH below the pI of the protein.

Techniques: Shear, Cell Culture, Purification, Chromatography, Enzyme-linked Immunosorbent Assay, Standard Deviation